Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes
نویسندگان
چکیده
منابع مشابه
Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes.
A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS2 region, restriction digestion with BstUI (CG/CG) and restriction fragment le...
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متن کاملMolecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)
BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2010
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.013870-0